Phage display is a powerful technique that has greatly accelerated progress in multiple fields, including drug discovery, structural biology, biomarker development, and protein domain engineering. This technology leverages bacteriophages-viruses that infect bacteria-to display a wide range of foreign peptides, proteins, or antibody fragments on their surfaces, allowing for the identification of optimal binding molecules. With contributions from instructors and former students from the "Antibody Engineering and Display Technologies" Cold Spring Harbor Laboratory course, this collection features expert reviews and detailed laboratory protocols covering various principles and applications of phage display, and represents an essential resource for those using phage display in molecular biology and biotechnology.